SzSA YearBook 2016/17
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SZENT-GYÖRGYI MENTORS<br />
GYÖRGY PÓSFAI<br />
Institute of Biochemistry,<br />
Biological Research Center of the<br />
Hungarian Academy of Sciences<br />
Address: Temesvári krt. 62., H-6726 Szeged, Hungary<br />
E: posfai.gyorgy@brc.mta.hu<br />
T: +36 62/599-778<br />
RESEARCH AREA<br />
Synthetic biology aims at constructing life forms that do<br />
not exist in nature. The motivation is two-fold: better understanding<br />
of the living cell, and creating useful cells for<br />
industrial purposes.<br />
We are currently focusing on the large-scale remodeling of<br />
bacterial genomes to understand principles shaping genome<br />
content and architecture, and to construct cells that<br />
serve as improved model organisms for research, and as a<br />
cellular chassis for biotechnological applications. We are<br />
particularly interested in:<br />
– Developing fast and effective genome engineering protocols<br />
– Minimizing and shuffling the genomes of E. coli strains<br />
– Studying the effect of specific genomic elements on genome<br />
architecture and evolution.<br />
TECHNIQUES AVAILABLE IN THE LAB<br />
Synthetic and molecular biology techniques, microbiological<br />
protocols, assembly and multiplex modification<br />
of genes and genomes, genome analysis.<br />
SELECTED PUBLICATIONS<br />
Kolisnychenko, V., Plunkett, GIII., Herring, C.D., Fehér, T., Pósfai,<br />
J., Blattner, F.R., Pósfai, G. (2002) Engineering a reduced<br />
E. coli genome. Genome Res 12: 640-647.<br />
Pósfai, G., Plunkett, G.3rd., Fehér, T., Frisch, D., Keil, G., Umenhoffer,<br />
K, Kolisnychenko, V., Stahl, B, Arruda, M., Sharma, S.S.,<br />
Burland, V., Harcum, S.W., Blattner, F.R. (2006) Emergent<br />
properties of reduced-genome Escherichia coli. Science<br />
312: 1044-1046.<br />
Fehér, T., Papp, B., Pál, C., Pósfai, G. (2007) Systematic genome<br />
reductions: Theoretical and experimental aspects.<br />
Chem Rev 107: 3498-3513.<br />
Csörgő, B., Fehér, T., Timár, E., Blattner, F.R., Pósfai, G. (2012)<br />
Low-mutation-rate, reduced-genome Escherichia coli: An<br />
improved host for faithful maintenance of engineered genetic<br />
constructs. Microb Cell Fact 11: 11.<br />
Fehér, T., Burland, V., Pósfai, G. (2012) In the fast lane: Large-scale<br />
bacterial genome engineering. J Biotechnol 160:<br />
72-79.<br />
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