XII - 12th International Symposium - Digestive Physiology of Pigs

Digestive
Physiology
of Pigs
subtilis C-3102 (C-3102), on health and productivity of
sows and their litters. The study was performed in a farrowto-finish
farm with a capacity of 350 sows and its own feed
mill, using an all-in-all-out batch management system.
Following weaning of the previous litter, sows were housed
in individual crates for 30 d, until served and pregnancy
confirmed. Subsequently, weekly batches of 14-15 sows at
the same gestation stage were loose-housed in a pen with
individual feeders until 103-110 d of gestation, then moved
to a farrowing room until weaning of piglets at approximately
26 d of age. Four batches of sows were studied throughout
pregnancy and lactation. Of these, two batches of sows
(T2) were fed a top-dressing supplying 3 x10 5 cfu C-3102
per gram complete feed, and two control batches (T1) were
fed the basal top-dressing free of probiotic. The offspring
of the T1 control group were offered T1 basal creep feed,
while piglets of the T2 group were offered basal feed
supplemented with C-3102 supplying 3 x10 5 cfu C-3102 per
gram complete feed. Each dam with her litter represented
an experimental unit (replicate). Treatment groups (T1 and
T2) were similar with respect to sow age/parity distribution.
During lactation, weight loss of T2 sows was significantly
lower, while mean weaning weight and growth, birth to
weaning, of T2 piglets were significantly improved. The
positive effect of C-3102 on sow condition during lactation,
as evidenced by reduced weight loss, may improve sow
milk production and conception rate. By weaning, T2 piglets
were on average 0.5 kg heavier than T1 control piglets,
possibly due to indirect (through the dam), or direct (through
the creep feed) effects of the probiotic.
Key words: sows, Bacillus subtilis C-3102, creep feed
1019 G protein-coupled receptor120 (GPR120) transcription
in intestinal epithelial cells are significantly
affected by bacteria belonging to the Bacteroides,
Proteobacteria, and Firmicutes phyla. M. Fredborg, P.
K. Theil, B. B. Jensen, and S. Purup,* Aarhus University,
Denmark.
Free fatty acids (FFAs) are produced in the intestine by
microbial fermentation. Recently, a family of G proteincoupled
receptors (GPR) acting as FFA transporters has
been reported; including GPR120 which is expressed by
intestinal epithelial cells and has been reported to function
as a control point for anti-inflammatory effects. The aim
of the present study was to evaluate whether 12 selected
intestinal bacteria, representing the 3 major phyla present
in the intestine, affects intestinal epithelial cell GP120transcription
Caco-2 intestinal epithelial cells were cultured
on filter inserts for 21 d until fully differentiated. Supernatants
of the 12 bacteria were added to cell culture medium at
the apical side in a cell:bacteria ratio of 1:200. After 4 h
incubation, changes in cellular transcription of GPR120
by bacterial supernatant were examined using real time
reverse transcriptase polymerase chain reaction (RT-PCR).
Supernatants from 6 of the 12 bacteria analyzed influenced
the mRNA level of GPR120 significantly (P < 0.05) compared
with cells without added bacteria. A significant increase in
cellular GPR120 mRNA was observed by E. faecium, L.
reuteri, L. salivarius, E. coli K-12, B. fragilis and F. prausnitzii.
The greatest increase was observed by the latter 2, which
XII INTERNATIONAL SYMPOSIUM ON
DIGESTIVE PHYSIOLOGY OF PIGS
45
Session I
was observed to increase cellular GPR120 mRNA level by
>2.1 fold. Intriguingly, these bacteria are categorized as
either probiotics or bacteria capable of introducing an antiinflammatory
effect. The beneficial effect of these bacteria
may very well be mediated by regulation of GPR120. The
regulation of GPR120 by intestinal microbiota represents
a direct signaling pathway for gut bacteria to affect host
health and metabolism.
Key words: bacteria, GPR120, intestinal epithelial cells
1020 Intestinal delivery of probiotics bacteria protected
with succinylated β-lactoglobulin tablets and
their effects on pig intestinal microbiota. I. Paquette* 1,2 ,
J.-P. Brousseau 2 , G. Talbot 2 , M. Lessard 2 , R. Caillard 1 , and
M. Subirade 1 , 1 Université Laval, Département des sciences
et technologie des aliments, Québec, Québec, Canada,
2 Dairy and Swine R & D Centre, Agriculture and Agri-Food
Canada, Sherbrooke, Québec, Canada.
This study was performed to evaluate the potential of
succinylated β-lactoglobulin (β-lg) tablets to protect 2
probiotics, Lactobacillus helveticus (Lh) and Bifidobacterium
longum (Bl) from in vitro gastric condition and to determine,
in vivo, the influence of delivering protected probiotics in
succinylated β-lg tablets on intestinal microbiota. To realize
in vivo study, 48 weaned piglets of 28 d-old, were divided
into 3 groups and received one of the following tablets
daily: Group 1) succinylated β-lg tablets without probiotics,
Group 2) non-succinylated β-lg tablets with 10 9 cfu of Lh
and Bl, Group 3) succinylated β-lg tablets with 10 9 cfu of
Lh and Bl. After 14 and 28 d of treatment, 8 piglets per
group were euthanized and colon contents were sampled
to characterize bacterial population (selective medium
and T-RFLP) and confirm the presence of Bl by qPCR. In
vitro study showed that after 1 h incubation in simulated
gastric condition, more than 10 9 cfu of each strain were
still viable when incorporated in succinylated β-lg tablets.
In vivo, piglets receiving protected probiotics had higher (P
≤ 0.05) bifidobacteria counts in colon content than groups
1 and 2 after 28 d. These results suggest that the use of
succinylated β-lg tablets helped maintain the viability of
the probiotics as they were delivered into the intestine.
Analysis of colonic microbiota T-RFLP profiles showed that
the relative abundance of bacterial population belonging
to Ruminococcaceae was significantly different between
treatments (P ≤ 0.05), with a higher abundance for group
3. However, diversity indices (Evenness and Shannon)
calculated from T-RFLP profiles revealed no major influence
of our treatments on the colonic microbiota. Using specific
primers for Bl, qPCR assays showed its presence in the
colon of animals receiving tablets containing probiotics
(groups 2 and 3). In conclusion, these results suggest that
the protected probiotics were delivered alive in the intestine
and their daily administration can modulate specific bacterial
populations without affecting the overall microbiota.
Key words: probiotics, microbiota, encapsulation
1021 Dietary supplementation with alkaline phosphatase
affects intestinal microbial populations of nursery
pigs. M. H. Rostagno* 1 , J. Ferrel 2 , J. S. Radcliffe 3 , and B. T.