Pentose-Fermenting Zymomonas mobilis - EERE - U.S. Department ...

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Cultivation Conditions • Media: − RM: 10 g/L YE, 2 g/L KH2PO4, 2% glucose (desired sugars) or Clarified CSL (cCSL) (1%) + sugars − Fermentation Media using hydrolysate : cCSL (1%) + Hydrolysate, overlimed or neutralized supplemented with extra glucose. • Inoculum Size: 0.03-0.25 OD @600 nm (0.01 - 0.085 g cell/L) • Fermentor: pH 5 or pH 6 controlled with KOH (2N) • Temperature at 30°C or 37°C • No aeration
Xylose, Ethanol (g/L) 120 100 80 60 40 20 0 Z. <strong>mobilis</strong> 8b on RM 10% xylose Xylose 103 g/l Ethanol 48 g/l 0 20 40 60 80 100 120 Time (hr) 1.2 1.0 0.8 0.6 0.4 0.2 0.0 DCM (g/L) Y m: 0.47 g/g Y p: 0.46 g/g q E: 1.3 g/g/h Vol. Prod.: 0.4 g/l/h
Page 1 and 2: Zymomonas mobilis Special Topics Se
Page 3 and 4: Metabolic Engineering for Pentose F
Page 5: Highlights of Zymomonas mobilis •
Page 9 and 10: Corn Stover Hydrolysates Compound C
Page 11 and 12: Glucose, Xylose, Ethanol (g/l) 50 4
Page 13 and 14: Glucose, Xylose, Ethanol (g/l) Ferm
Page 15 and 16: Future Improvements • Ferment all
Page 17 and 18: Cytoplasmic membrane Is Sugar Trans

Pentose-Fermenting Zymomonas mobilis - EERE - U.S. Department ...

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