Pentose-Fermenting Zymomonas mobilis - EERE - U.S. Department ...

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Metabolic Engineering Tools • Transcriptomics � Examine gene expression at global level • Metabolomics � Examine metabolites at global level • High-throughput Enzyme Assays � Compare strains � Compare activities at different stages of growth � Compare activities in different growth conditions � Develop improved enzymes • Mathematical approaches − Metabolic flux analysis − Kinetic modeling
Cytoplasmic membrane Is Sugar Transport limiting? Transporter protein in xylose out xylose Facilitated Diffusion Transport No energy required
Page 1 and 2: Zymomonas mobilis Special Topics Se
Page 3 and 4: Metabolic Engineering for Pentose F
Page 5 and 6: Highlights of Zymomonas mobilis •
Page 7 and 8: Xylose, Ethanol (g/L) 120 100 80 60
Page 9 and 10: Corn Stover Hydrolysates Compound C
Page 11 and 12: Glucose, Xylose, Ethanol (g/l) 50 4
Page 13 and 14: Glucose, Xylose, Ethanol (g/l) Ferm
Page 15: Future Improvements • Ferment all

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