Pentose-Fermenting Zymomonas mobilis - EERE - U.S. Department ...

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Concentration (g/L) Adaptation of <strong>Zymomonas</strong> 39676(pZB4L) to above 60% (v/v) OL Hydrolyzate 50 40 30 20 10 0 CSL=2% (v/v) D = 0.015 (1/hr) Strain Improvement through Adaptation Feed Mixture: Glucose (8 g/L) + Xylose (40 g/L) T=33C, pH=5.8 glucose xylose ethanol % hydrolyzate 0 10 20 30 40 50 60 70 Time (Days) 80 70 60 50 40 30 20 10 0 Hydrolysate (%, V/V) Concentration (g/L) 50 40 30 20 10 0 Comparison of Adapted and Parent Strain 39676(pZB4L) Grown on cCSL (2%), 60% Sawdust Hydrolysate at pH 5.8, T 30°C, Initial Sugar Conc. (G:X 1%:5%) Glucose Xylose Ethanol Ethanol Adapted Parent Ethanol
Future Improvements • Ferment all biomass sugars (including mannose and galactose) • Improve pentose fermentation yield and productivity • Improve tolerance to hydrolysate toxicity
Page 1 and 2: Zymomonas mobilis Special Topics Se
Page 3 and 4: Metabolic Engineering for Pentose F
Page 5 and 6: Highlights of Zymomonas mobilis •
Page 7 and 8: Xylose, Ethanol (g/L) 120 100 80 60
Page 9 and 10: Corn Stover Hydrolysates Compound C
Page 11 and 12: Glucose, Xylose, Ethanol (g/l) 50 4
Page 13: Glucose, Xylose, Ethanol (g/l) Ferm
Page 17 and 18: Cytoplasmic membrane Is Sugar Trans

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