CSA-Journal-2016-04

Fig. 13 Seedling of Cym. goeringii 1 year after
deflasking
Fig. 14 Album form of Cym. tortisepalum in
flower.
tiation in Jensoa cymbidium tissue culture.
It consists of V&W salts, 3 g/l peptone,
micro elements and vitamins as for MS media,
25 g/l sucrose, 2 mg/l 6-Benzylaminopurine
(BAP), and 0.2 mg/l 1-Naphtaleneacetic
acid (NAA). The best replating
medium that leads to good mycorrhizome
development is a modified Knudson-C (i.e.
with 1.5 g/l peptone, 25 g/l banana, 50 g/l
potato, 10 g/l maltodextrin, no PGR’s, 2 g/l
activated charcoal, 15 g/l sucrose). Sowing
and first replating happen in constant
darkness at 20 °C. When sufficient bundles
of mycorrhizomes are developed they can
be separated, placed on the shoot initiation
medium, and placed under 12 hours light
at 25 °C. The best medium for us proved to
be a modified Hyponex medium (2 g/l Hyponex,
3 g/l peptone, 250 mg/l Ca(NO3)2
x 4 H2O, 10 mg/l BAP, 1 mg/l NAA, 150
ml/l coconut water, MS vitamins, 30 g/l sucrose).
With the aid of the 10 mg/l BAP
and 1 mg/l NAA most mycorrhizomes produce
shoots. On a Knudson-C modifica-
Fig. 15 Seedlings of Cym. tortisepalum album from
Figure 14 about 2 years after deflasking.
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