Mapping 250K/500K SNP assay

ELUTE THE PCR PRODUCTS
82 GeneChip ® Mapping 500K Assay Manual
To elute the PCR products:
1. When the wells are completely dry after the third wash, turn off
the vacuum.
2. Carefully remove the plate from the manifold and immediately:
A. Blot the bottom of the plate on a thick stack of clean absorbent
paper to remove any remaining liquid.
B. Dry the bottom of each well with an absorbent wipe.
Immediately blot the bottom of the plate and dry the bottom of
each well. Any remaining liquid will quickly seep back into the
wells.
3. Aliquot 5 mL RB Buffer to a solution basin.
4. Using a 12-channel P200 pipette, add 45 µL RB buffer to each
well of the plate.
5. Tightly seal the plate.
6. Load the plate onto a Jitterbug plate shaker.
7. Set the Jitterbug to setting 5 and moderately shake the plate for
10 minutes at room temperature.
This setting (approximately 1000 rpm) allows as much movement
as possible without losing liquid to the sides of the wells and film.
8. Transfer 45 µL of each eluted sample from the Clontech Clean-Up
Plate to the corresponding well of a fresh 96-well plate following
these guidelines:
• Use a 12-channel P200 pipette set to 60 µL.
• Tilt the Clontech Clean-Up Plate at a 30 to 45 degree angle to
move the liquid to one side of the well.
Optional: use a plate support to keep the plate tilted at an angle
(Well Plate Stand: Diversified Biotech, P/N WPST-1000).
• Pipette up and down 3 to 4 times before removing and
transferring the eluate to a fresh 96-well reaction plate.