Mapping 250K/500K SNP assay

Figure 4.4
Pooling PCR Products Onto the Clean-Up Plate
PURIFY THE PCR PRODUCTS
80 GeneChip ® Mapping 500K Assay Manual
P1, P2 and P3 = PCR Product Plates
P1 P2 P3
CUP - BL
Three water washes must be performed to properly purify the PCR
PCR
products. Be sure to completely dry the membrane after the third
wash.
To purify the PCR products:
Clean-Up Plate
= Pooled PCR product
from row A of plate
BL = bottom left
Transfer and pool each PCR product from plates P1, P2 and P3 to the corresponding well of the
Clean-Up Plate. For example, transfer and pool the PCR product from well A1 of plates P1, P2
and P3 to the corresponding row and well on the Clean-Up Plate.
1. Load the Clontech Clean-Up Plate with samples onto the
manifold.
2. Cover the plate to protect the samples from environmental
contaminants.
For example, you can use the lid from a pipette tip box. Do not put
a plate seal on the wells containing sample.