Mapping 250K/500K SNP assay

POOL THE PCR PRODUCTS
chapter 4 | 96-Well Plate Protocol 79
To avoid piercing the Clean-Up Plate membrane, do not pipette up
and down in the plate, and do not touch the bottom of the plate.
Be very careful when pooling the third set of PCR products, as the
wells are very full. Avoid cross-contaminating neighboring wells
with small droplets.
Also, pipette very carefully to avoid the formation of air bubbles. Air
bubbles will slow drying.
Working one row at a time, pool the PCR products as follows:
1. Cut the adhesive film from the first row of each reaction plate.
2. Using a 12-channel P200 pipette, transfer and pool the samples
from the same row and well of each PCR product plate to the
corresponding row and well of the Clean-Up Plate.
Example (Figure 4.4 on page 80): transfer each sample from row A
of plates P1, P2 and P3 to the corresponding wells of row A on the
Clean-Up Plate.
To avoid piercing the membrane, do not pipette up and down in
the Clean-Up Plate.
3. Change your pipette tips.
Be sure to change pipette tips after each of the three corresponding
rows of sample are pooled onto the Clean-Up Plate.
4. Repeat these steps until all of the PCR products are pooled.
5. Examine the three PCR product plates to be sure that the full
volume of each well was transferred and that the plates are empty.
The final volume in each well on the Clontech Clean-Up Plate
should be approximately 320 µL.