Mapping 250K/500K SNP assay

78 GeneChip ® Mapping 500K Assay Manual
ADD DILUTED EDTA TO THE PCR PRODUCTS
To add diluted EDTA to the PCR products:
1. Add 3 mL of diluted EDTA (0.1M) to a solution basin.
2. Using a 12-channel P20 pipette, aliquot 8 µL of diluted EDTA to
each well with PCR product on each PCR product plate.
3. Tightly seal each plate.
PREPARE THE CLEAN-UP PLATE
4. Vortex the center of each plate at high speed for 3 sec.
5. Spin down each plate at 2000 rpm for 30 sec.
6. Place each plate back in a plate holder.
Follow the steps as described below. Consult the Clontech Clean-Up
Plate Handbook for the general procedure.
To prepare the Clean-Up Plate:
1. Label the plate to indicate its orientation CUP BL (Clean-Up Plate
bottom left).
2. If not processing a full plate of samples, cover the wells that will
not be used with adhesive film as follows:
A. Apply pressure around the edges of the plate to make the film
stick.
B. Cut the film between the used and unused wells.
C. Remove the portion that covers the wells you want to use.