Mapping 250K/500K SNP assay

REAGENTS REQUIRED
76 GeneChip ® Mapping 500K Assay Manual
** Use only the PCR plate, adhesive film and thermal cyclers listed
in Table 4.1 on page 40.
The following reagents are required for this stage. Refer to
Appendix A, Reagents, Equipment, and Consumables Required for 96-Well
Plate Protocol for vendor and part number information. The amounts
listed are sufficient to process one full 96-well reaction plate.
Table 4.20
Reagents Required for Stage 5: PCR Product Purification and Elution
Quantity Reagent
1 Clean-Up Plate (Clontech)
3 mL EDTA, diluted to 0.1M (working stock is 0.5 M, pH 8.0)
5 mL RB Buffer
75 mL AccuGENE ® water, molecular biology-grade
IMPORTANT INFORMATION ABOUT THIS STAGE
To help ensure the best results, carefully read the information below
before you begin this stage of the protocol.
• The working stock of EDTA must be diluted to 0.1 M before use.
•The AccuGENE® water listed in Appendix A must be used for this
stage. Using in-house ddH 2O is not acceptable and can negatively
impact downstream stages, particularly Stage 7: Fragmentation. The
fragmentation reaction is very sensitive to pH and metal ion
contamination.
• To avoid cross-contamination and the introduction of air bubbles,
pipette very careful when pooling the three PCR reactions for each
sample onto the Clontech Clean-Up Plate.
• Maintain the vacuum at 600 mbar.