Mapping 250K/500K SNP assay

74 GeneChip ® Mapping 500K Assay Manual
Stage 5: PCR Product Purification and Elution
ABOUT THIS STAGE
During this stage, you will:
• Add diluted EDTA to each PCR product
• Pool each corresponding PCR product back to a single reaction on a
Clontech Clean-Up Plate (Figure 4.4 on page 80).
• Place the Clean-Up Plate onto a manifold and concentrate the PCR
products.
• Wash the PCR products three times while still on the manifold
using AccuGENE® water.
• Elute the PCR products using RB Buffer and transfer them to a new
96-well plate.
LOCATION AND DURATION
• Main Lab
• Hands-on time: 1 hour
• Initial dry down: 1.5 to 2 hours
• First two water washes: 15 to 20 minutes each
• Third water wash: 45 to 75 minutes
INPUT REQUIRED FROM PREVIOUS STAGE
The input required from Stage 4: PCR is:
Quantity Item
3 Plates of PCR product in cooling chambers on ice.