Mapping 250K/500K SNP assay

70 GeneChip ® Mapping 500K Assay Manual
LOAD PCR PLATES ONTO THERMAL CYCLERS
PCR protocols for the MJ Tetrad PTC-225 and Applied Biosystems
thermal cyclers are different. See Table 4.17 and Table 4.18 below.
Location
Main Lab
Procedure
To load the plates and run the 500K PCR program:
1. Transfer the reaction plates to the Main Lab.
2. Ensure that the thermal cycler lids are preheated.
The block should be at room temperature.
3. Load each reaction plate onto a thermal cycler.
4. Run the 500K PCR program.
The program varies depending upon the thermal cyclers you are
using. See Table 4.17 for Applied Biosystems thermal cyclers and
Table 4.18 for Bio-Rad thermal cyclers.
If using GeneAmp® PCR System 9700 thermal cyclers, be sure the
blocks are silver or gold-plated silver. Do NOT use thermal cyclers
with aluminum blocks. It is not easy to visually distinguish between
silver and aluminum blocks.