Mapping 250K/500K SNP assay

66 GeneChip ® Mapping 500K Assay Manual
4. Prepare the Ligation Stage plate as follows:
A. Vortex the center of the plate at high speed for 3 sec.
B. Spin down the plate at 2000 rpm for 30 sec.
C. Label the plate Lig.
D. Place back in the cooling chamber on ice.
5. To prepare the reagents:
A. Vortex at high speed 3 times, 1 sec each time (except for the
enzyme).
B. Pulse spin for 3 sec.
C. Place in a cooling chamber.
ADD DNA TO THE REACTION PLATES
Preheat the Thermal Cycler Lids (Main Lab)
Have someone in the Main Lab power on the thermal cyclers to be used
for the PCR to preheat the lids. The lids must be preheated before
loading samples; leave the blocks at room temperature.
If you are preparing the plates for PCR, it is best not to go from the
Pre-PCR Room or Staging Area to the Main Lab and then back again.
To add DNA to the reaction plates:
1. Working one row at a time and using a 12-channel P20 pipette,
transfer 10 µL of sample from each well of the Ligation Plate to the
corresponding well of each reaction plate.
Example (Figure 4.2 on page 67): Transfer 10 µL of sample from
each well of row A on the Ligation Plate to the corresponding wells
of row A on reaction plates P1, P2 and P3.
2. Seal each plate with adhesive film, and leave in cooling chambers
on ice.