Mapping 250K/500K SNP assay

About Controls
chapter 4 | 96-Well Plate Protocol 65
A PCR negative control can be included in the experiment to assess
the presence of contamination. Refer to Chapter 2 and Chapter 7 for
more information.
PREPARE THE REAGENTS, CONSUMABLES AND OTHER COMPONENTS
Thaw Reagents and Ligation Stage Plate
To thaw the reagents and Ligation Stage Plate:
1. Allow the following reagents to thaw on ice.
• TITANIUM Taq PCR Buffer
• dNTPs
• PCR Primer 002
Leave the TITANIUM Taq DNA Polymerase at –20 °C until ready to
use.
2. If the Ligation Stage plate was frozen, allow to thaw in a cooling
chamber on ice.
Prepare Your Work Area (Pre-PCR Clean Area)
To prepare the work area:
1. Place two double or four single cooling chambers and one cooler on
ice (Figure 4.1 on page 39).
2. Label the following, then place in a cooling chamber:
• Three 96-well reaction plates labeled P1, P2, P3; see Figure 4.2
on page 67
• A 50 mL Falcon tube labeled PCR MM
3. Place on ice:
• AccuGENE® water
• GC-Melt
• Solution basin