Mapping 250K/500K SNP assay

64 GeneChip ® Mapping 500K Assay Manual
GELS AND RELATED MATERIALS REQUIRED
The following gels and related materials are required for this stage.
Refer to Appendix A, Reagents, Equipment, and Consumables Required for
96-Well Plate Protocol for vendor and part number information. The
amounts listed are sufficient to process one full 96-well reaction plate.
Table 4.15
Gels and Related Materials Required for Stage 4: PCR
Quantity Reagent
50 µL DNA Marker
13 Gels, 2% TBE
As needed Gel loading solution
3 Plates, 96-well reaction
IMPORTANT INFORMATION ABOUT THIS STAGE
To help ensure the best results, carefully read the information below
before you begin this stage of the protocol.
• Make sure the ligated DNA was diluted to 100 µL with
AccuGENE® water.
• Prepare PCR Master Mix immediately prior to use, and prepare in
Pre-PCR Clean room. To help ensure the correct distribution of
fragments, be sure to add the correct amount of primer to the master
mix. Mix the master mix well to ensure the even distribution of
primers.
• Set up the PCRs in PCR Staging Area.
• To ensure consistent results, take 3 µL aliquots from each PCR to
run on gels before adding EDTA.