Mapping 250K/500K SNP assay

REAGENTS REQUIRED
chapter 4 | 96-Well Plate Protocol 63
** Use only the PCR plate, adhesive film and thermal cyclers listed
in Table 4.1 on page 40.
The following reagents are required for this stage. Refer to
Appendix A, Reagents, Equipment, and Consumables Required for 96-Well
Plate Protocol for vendor and part number information. The amounts
listed are sufficient to process one full 96-well reaction plate.
Table 4.14
Reagents Required for Stage 4: PCR
Quantity Reagent
15 mL AccuGENE® water, molecular biology-grade
875 µL (2 vials) PCR Primer 002 (100 µM)
The following reagents from the Clontech TITANIUM DNA Amplification Kit
1.28 mL dNTPs (2.5 mM each)
1 mL GC-Melt (5M)
100 µL TITANIUM Taq DNA Polymerase (50X)
600 µL TITANIUM Taq PCR Buffer (10X)