Mapping 250K/500K SNP assay

DILUTE THE SAMPLES
To dilute the samples:
chapter 4 | 96-Well Plate Protocol 59
8. Place the AccuGENE® Water on ice 20 minutes prior to use.
1. When the 500K Ligate program is finished, remove the plate and
spin it down at 2000 rpm for 30 sec.
2. Place the plate in a cooling chamber on ice.
3. Dilute each reaction as follows:
500K Ligate Program
Temperature Time
16ºC 180 minutes
70ºC 20 minutes
4ºC Hold
A. Pour 10 mL AccuGENE® water into the solution basin.
B. Using a 12-channel P200 pipette, add 75 µL of the water to
each reaction.
The total volume in each well is 100 µL.
Reagent Volume/Sample
Ligated DNA 25 µL
AccuGENE® water 75 µL
Total 100 µL
4. Seal the plate tightly with adhesive film.
5. Vortex the center of the plate at high speed for 3 sec.
6. Spin down the plate at 2000 rpm for 30 sec.
It is crucial to dilute the ligated DNA with AccuGENE® water prior
to PCR.