Mapping 250K/500K SNP assay

52 GeneChip ® Mapping 500K Assay Manual
Reagent Volume/Sample
Genomic DNA (50 ng/µL) 5 µL
Digestion Master Mix 14.75 µL
Total Volume 19.75 µL
3. Seal the plate tightly with adhesive film.
4. Vortex the center of the plate at high speed for 3 sec.
5. Spin down the plate at 2000 rpm for 30 sec.
6. Ensure that the lid of thermal cycler is preheated.
7. Load the plate onto the thermal cycler and run the 500K Digest
program.
500K Digest Program
Temperature Time
37ºC 120 minutes
65ºC 20 minutes
4ºC Hold
8. When the program is finished, remove the plate and spin it down
at 2000 rpm for 30 sec.
9. Do one of the following:
• If proceeding directly to the next step, place the plate in a
cooling chamber on ice.
• If not proceeding directly to the next step, store the samples at
–20 °C.