Mapping 250K/500K SNP assay

Table 4.7
Nsp I Digestion Master Mix
ADD DIGESTION MASTER MIX TO SAMPLES
To add Digestion Master Mix to samples:
chapter 4 | 96-Well Plate Protocol 51
Reagent 1 Sample 96 Samples
(15% extra)
AccuGENE® Water 11.55 µL 1275.1 µL
NE Buffer 2 (10X) 2 µL 220.8 µL
BSA (100X; 10 mg/mL) 0.2 µL 22.1 µL
Nsp I (10 U/µL) 1 µL 110.4 µL
Total 14.75 µL 1628.4 µL
Table 4.8
Sty I Digestion Master Mix
Reagent 1 Sample 96 Samples
(15% extra)
AccuGENE® Water 11.55 µL 1275.1 µL
NE Buffer 3 (10X) 2 µL 220.8 µL
BSA (100X; 10 mg/mL) 0.2 µL 22.1 µL
Sty I (10 U/µL) 1 µL 110.4 µL
Total 14.75 µL 1628.4 µL
1. Using a single channel P200 pipette, aliquot 135 µL of Digestion
Master Mix to each tube of the strip tubes labeled Dig.
2. Using a 12-channel P20 pipette, add 14.75 µL of Digestion Master
Mix to each DNA sample in the cooling chamber on ice.
The total volume in each well is now 19.75 µL.