Mapping 250K/500K SNP assay

ALIQUOTING PREPARED GENOMIC DNA
To aliquot the prepared genomic DNA:
chapter 4 | 96-Well Plate Protocol 45
1. Vortex the plate of genomic DNA at high speed for 10 sec, then
spin down at 2000 rpm for 30 sec.
2. Aliquot 5 µL of each DNA to the corresponding wells of a 96-well
reaction plate.
5 µL of the 50 ng/µL working stock is equivalent to 250 ng
genomic DNA per well.
For this protocol, one plate is required to process Nsp samples; a
second plate is required to process Sty samples. Do not process Nsp
and Sty samples on the same day.
3. If continuing immediately to the next stage, place the plate with
prepared genomic DNA in a double cooling chamber on ice.
Otherwise, seal each plate with adhesive film.
WHAT YOU CAN DO NEXT
Do one of the following:
• Proceed to the next stage, processing one plate of samples, one
enzyme at a time.
• Store the sealed plates of diluted genomic DNA at –20 °C.