Mapping 250K/500K SNP assay

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Equipment and Consumables Required 54
Reagents Required 55
Important Information About This Procedure 55
Prepare the Reagents, Consumables and Other Components 55
Prepare the Ligation Master Mix 57
Add Ligation Master Mix to Reactions 58
Dilute the Samples 59
What You Can Do Next 60
STAGE 4: PCR 61
About this Stage 61
Location and Duration 61
Input Required from Previous Stage 61
Equipment and Materials Required 62
Reagents Required 63
Gels and Related Materials Required 64
Important Information About This Stage 64
Prepare the Reagents, Consumables and Other Components 65
Add DNA to the Reaction Plates 66
Prepare the PCR Master Mix 68
Add PCR Master Mix to Samples 69
Load PCR Plates Onto Thermal Cyclers 70
Running Gels 72
What You Can Do Next 73
STAGE 5: PCR PRODUCT PURIFICATION AND ELUTION 74
About this Stage 74
Location and Duration 74
Input Required from Previous Stage 74
Equipment and Consumables Required 75
Reagents Required 76
Important Information About This Stage 76
Prepare the Reagents, Consumables and Other Components 77
Add Diluted EDTA to the PCR Products 78
Prepare the Clean-Up Plate 78
Pool the PCR Products 79
Purify the PCR Products 80
Elute The PCR Products 82
What You Can Do Next 83
STAGE 6: QUANTITATION AND NORMALIZATION 84
About this Stage 84
Location and Duration 84