Mapping 250K/500K SNP assay

appendix D | Reagents, Instruments, and Supplies Required for Low Throughput Protocol 293
Table D.6
Reagents, Equipment and Supplies Not Supplied by Affymetrix
Assay Step Reagents Equipment
Genomic DNA
Preparation
Restriction Enzyme
Digestion
Reduced EDTA TE Buffer (10 mM Tris HCL, 0.1 mM
EDTA, pH 8.0): TEKnova, P/N T0223
Reference Genomic DNA, 103 (50 ng/µL):
Affymetrix * , P/N 900421, available in Box 2 of
the GeneChip ® Mapping 250K Assay Kits:
Nsp 30 Rxn Kit P/N 900766
Nsp 100 Rxn Kit P/N 900753
Sty 30 Rxn Kit P/N 900765
Sty 100 Rxn Kit P/N 900754
250 ng Genomic DNA 50 ng/µL working stock/array
Sty I (10,000 U/mL): New England Biolabs
(NEB), P/N R0500S (if running Sty Array)
containing:
NE Buffer 3: New England Biolabs (NEB),
(P/N B7003S to order separately)
BSA (Bovine Serum Albumin): New England
Biolabs (NEB), (P/N B9001S to order
separately)
Nsp I (10,000 U/mL): New England Biolabs
(NEB), P/N R0602L (if running Nsp Array)
containing:
NE Buffer 2: New England Biolabs (NEB),
(P/N B7002S to order separately)
H 2O (Molecular Biology Grade Water): BioWhittaker
Molecular Applications/Cambrex, P/N 51200
96-well plate: Bio-Rad, P/N MLP-9601; or Applied
Biosystems, P/N 403083
96-well Plate Clear Adhesive Films: Applied
Biosystems, P/N 4306311
8-Tube Strips, thin-wall (0.2 mL): Bio-Rad,
P/N TBS-0201; Strip of 8 caps: Bio-Rad,
P/N TCS-0801
Thermal Cycler