Mapping 250K/500K SNP assay

Table D.4
Reagents Supplied by Affymetrix - 100 reaction Kit
appendix D | Reagents, Instruments, and Supplies Required for Low Throughput Protocol 291
Affymetrix GeneChip ® Mapping 250K Sty Assay Kit P/N 900754
This kit includes sufficient reagent for 100 arrays.
Component Volume Concentration Description Box #
Adaptor, Sty I 75 µL 50 µM Two annealed oligonucleotides, specific
for ligation to Sty restriction site.
PCR Primer, 002 2 vials,
750 µL
each
Reference Genomic
DNA, 103
GeneChip ®
Fragmentation Reagent
10X Fragmentation
Buffer
GeneChip ® DNA
Labeling Reagent (30
mM)
Terminal
Deoxynucleotidyl
Transferase
5X Terminal
Deoxynucleotidyl
Transferase Buffer
Oligo Control Reagent,
0100 (OCR, 0100)
100 µM PCR primer, to amplify ligated genomic
DNA
30 µL 50 ng/µL Human genomic DNA (single source) 2
25 µL See label on tube DNase I enzyme, formulated to fragment
purified PCR amplicons
835 µL 10X Buffer for fragmentation reaction 3
200 µL 30 mM Biotin-labeled reagent for end-labeling
fragmented PCR amplicons
350 µL 30 U/µL Enzyme used to end-label fragmented
PCR amplicons with the GeneChip ® DNA
Labeling Reagent (30 mM)
2 vials,
700 µL
each
5X Buffer for labeling reaction 3
200 µL See protocol
Chapter 4 (high
throughput) or
Appendix C (low
throughput)
Mixture of five biotin-labeled
oligonucleotides, which hybridize to
control regions (gridding controls and
array controls) on the GeneChip ®
Mapping 250K Arrays.
1
1
3
3
3
3