Mapping 250K/500K SNP assay

290 GeneChip ® Mapping 500K Assay Manual
Table D.3
Reagents Supplied by Affymetrix - 30 reaction Kit
Affymetrix GeneChip ® Mapping 250K Sty Assay Kit P/N 900765
This kit includes sufficient reagent for 30 arrays.
Component Volume Concentration Description Box #
Adaptor, Sty I 25 µL 50 µM Two annealed oligonucleotides, specific
for ligation to Sty restriction site.
PCR Primer, 002 450 µL 100 µM PCR primer, to amplify ligated genomic
DNA
Reference Genomic
DNA, 103
GeneChip ®
Fragmentation Reagent
10X Fragmentation
Buffer
GeneChip ® DNA
Labeling Reagent
(30 mM)
Terminal
Deoxynucleotidyl
Transferase
5X Terminal
Deoxynucleotidyl
Transferase Buffer
Oligo Control Reagent,
0100 (OCR, 0100)
30 µL 50 ng/µL Human genomic DNA (single source) 2
25 µL See label on tube DNase I enzyme, formulated to fragment
purified PCR amplicons
250 µL 10X Buffer for fragmentation reaction 3
60 µL 30 mM Biotin-labeled reagent for end-labeling
fragmented PCR amplicons
105 µL 30 U/µL Enzyme used to end-label fragmented
PCR amplicons with the GeneChip ® DNA
Labeling Reagent (30 mM)
420 µL 5X Buffer for labeling reaction 3
60 µL See protocol
Chapter 4 (high
throughput) or
Appendix C (low
throughput)
Mixture of five biotin-labeled
oligonucleotides, which hybridize to
control regions (gridding controls and
array controls) on the GeneChip ®
Mapping 250K Arrays.
1
1
3
3
3
3