Mapping 250K/500K SNP assay

288 GeneChip ® Mapping 500K Assay Manual
Table D.2
Reagents Supplied by Affymetrix - 100 Reaction Kit
Affymetrix GeneChip ® Mapping 250K Nsp Assay Kit P/N 900753
This kit includes sufficient reagent for 100 arrays.
Component Volume Concentration Description Box #
Adaptor, Nsp I 75 µL 50 µM Two annealed oligonucleotides,
specific for ligation to Nsp restriction
site.
PCR Primer, 002 2 vials,
750 µL
each
Reference Genomic DNA,
103
GeneChip ®
Fragmentation Reagent
100 µM PCR primer, to amplify ligated genomic
DNA
30 µL 50 ng/µL Human genomic DNA (single source) 2
25 µL See label on tube DNase I enzyme, formulated to
fragment purified PCR amplicons
10X Fragmentation Buffer 835 µL 10X Buffer for fragmentation reaction 3
GeneChip ® DNA Labeling
Reagent (30 mM)
Terminal
Deoxynucleotidyl
Transferase
5X Terminal
Deoxynucleotidyl
Transferase Buffer
Oligo Control Reagent,
0100 (OCR, 0100)
200 µL 30 mM Biotin-labeled reagent for end-labeling
fragmented PCR amplicons
350 µL 30 U/µL Enzyme used to end-label fragmented
PCR amplicons with the GeneChip ®
DNA Labeling Reagent (30 mM)
2 vials,
700 µL
each
5X Buffer for labeling reaction 3
200 µL See protocol
Chapter 4 (high
throughput) or
Appendix C (low
throughput)
Mixture of five biotin-labeled
oligonucleotides, which hybridize to
control regions (gridding controls and
array controls) on the GeneChip ®
Mapping 250K Arrays.
1
1
3
3
3
3