Mapping 250K/500K SNP assay

HYBRIDIZATION PROCEDURE
appendix C | Low Throughput Protocol 279
Gloves, safety glasses, and lab coats must be worn when preparing
the hybridization cocktail. Please consult the appropriate MSDS for
reagent storage and handling requirements.
It is important to allow the arrays to equilibrate to room
temperature completely. Specifically, if the rubber septa are not
equilibrated to room temperature, they may be prone to cracking,
which can lead to leaks.
DMSO is light sensitive and must be stored in a dark glass bottle.
Do not store in a plastic container.
1. Prepare the Hybridization Cocktail Master Mix in the order shown
in Table C.14. For multiple samples, prepare 5% excess.
Table C.14
Hybridization Cocktail Master Mix
Reagent 1X Final Conc. in Sample
MES (12X; 1.22 M) 12 µL 0.056 M
DMSO (100%) 13 µL 5.0%
Denhardt’s Solution (50X) 13 µL 2.50X
EDTA (0.5 M) 3 µL 5.77 mM
HSDNA (10 mg/mL) 3 µL 0.115 mg/mL
OCR, 0100 2 µL 1X
Human Cot-1 DNA ® (1 mg/mL) 3 µL 11.5 µg/mL
Tween-20 (3%) 1 µL 0.0115%
TMACL (5 M) 140 µL 2.69 M
Total 190 µL