Mapping 250K/500K SNP assay

LABELING PROCEDURE
MAIN LAB
appendix C | Low Throughput Protocol 275
Program the thermal cycler in advance. Switch on the thermal
cycler 10 minutes before reactions are ready so that the lid is
heated.
1. Prepare Labeling Mix as master mix ON ICE and vortex at
medium speed for 2 seconds (for multiple samples make a 5%
excess).
Table C.12
Reagent 1X Final Conc. in
Sample
5X TdT Buffer 14 µL 1X
GeneChip ® DNA Labeling Reagent (30 mM) 2 µL 0.857 mM
TdT (30 U/µL) 3.5 µL 1.5 U/µL
Total 19.5 µL
2. Aliquot 19.5 µL of Labeling Master Mix into the fragmentation
plate containing 50.5 µL of fragmented DNA samples as follows:
Table C.13
Reagent Volume/Rx
Fragmented DNA
(from Fragmentation step)
Labeling Mix 19.5 µL
Total 70 µL
50.5 µL Remaining fragmented DNA
is used for gel analysis