Mapping 250K/500K SNP assay

appendix C | Low Throughput Protocol 265
6. Wash the PCR products by adding 50 µL molecular biology grade
water and dry the wells completely (approximately 20 minutes).
Repeat this step 2 additional times for a total of 3 water washes.
7. Switch off vacuum source and release the vacuum.
8. Carefully remove the Clean-Up Plate from the vacuum manifold
and immediately:
A. Blot the plate on a stack of clean absorbent paper to remove any
liquid that might remain on the bottom of the plate
B. Dry the bottom of each well with an absorbent wipe.
To remove all remaining liquid, blot and wipe the bottom of the
Clean-up Plate immediately after taking it off the vacuum manifold.
Any water retained underneath the plate may be absorbed back
into the wells.
9. Add 45 µL RB buffer to each well. Cover the plate with PCR plate
cover film and seal tightly. Moderately shake the Clean-Up Plate
on a plate shaker, e.g., Jitterbug (Boekel Scientific, model
130000), for 10 minutes at room temperature.
10. Recover the purified PCR product to a fresh 96-well plate by
pipetting the eluate out of each well and transferring it to the
corresponding well in the fresh 96-well plate. For easier recovery
of the eluates, the plate can be held at a slight angle.