Mapping 250K/500K SNP assay

262 GeneChip ® Mapping 500K Assay Manual
GeneAmp ® PCR System 9700 Program
Specify 100 µL volume and maximum mode.
500K PCR Program for Geneamp ® PCR System 9700
Temperature Time Cycles
94ºC 3 minutes 1X
94ºC
60ºC
30 seconds
45 seconds }
30X
68ºC 15 seconds
68ºC 7 minutes 1X
4ºC HOLD
6. Run 3 µL of each PCR product mixed with 3 µL 2X Gel Loading
Dye on 2% TBE gel at 120V for 1 hour.
PCR products can be stored at –20°C if not proceeding to the next
step within 60 minutes.
Figure C.2
Typical example of PCR products run on 2% TBE agarose gel at 120V for 1 hour,
with average size between 200 and 1,100 bp