Mapping 250K/500K SNP assay

PROBLEMS AND SOLUTIONS
Table 6.3
Common error messages, their meanings, probable causes and solutions
chapter 6 | Fluidics Station Care and Maintenance 171
Error Message Problem Possible Cause Solution
Missing Fluid Error Cartridge not filling
completely with sample
solution or buffer during
initial stages of
hybridization wash or
staining protocol.
Cartridge not filling
completely with buffer
during wash script
System detects improper
conditions while filling.
Note where in protocol
error occurred.
Recovered less sample
than initial input during
Recover script.
Possible holes in the
septa of the cartridge.
Sample or staining
solution not in place
properly.
Insufficient volume of
sample or staining
solution (500 µL).
Blocked sampling tube
or line of the fluidics
station.
Failure of one of the
fluidics sensors.
Pump tubing stretched
too tightly around the
pump.
Buffer bottle empty.
Module not primed.
Missing or insufficient
stain or antibody in vial.
Wash empty.
Air bubbles in line.
Leaks.
Loose tubing
attachments inside the
fluidics station.
Run Recover script, and then
use another cartridge.
Run Recover script. Make
sure sample or stain vial is in
the sample holder.
Run Recover script. Add
more sample solution to the
sample vial.
Run Recover script. Run the
Clean or Prime script with
fresh deionized (DI) water to
flush out salt blockage.
Call Affymetrix Technical
Support for service.
Loosen the tubing clamps,
allow tubing to relax, close
the clamps.
Fill buffer bottles.
Prime module.
Identify if chip is filled
If important to recover fluid
in chip, then run Recovery
script, followed by Resume
function
If not important to recover
fluid in chip, run Resume
function
Call Affymetrix Technical
Support for service.