Mapping 250K/500K SNP assay

160 GeneChip ® Mapping 500K Assay Manual
5. Open GeneChip ® Operating Software (GCOS), Microarray Suite,
or the current version of the Affymetrix control software.
Click Run → Fluidics... from the menu. Alternatively, click the
down arrow on the Protocol list on the toolbar. The protocol
window appears (Figure 6.3).
Figure 6.3
The Fluidics Station protocol window: select all modules.
6. Choose the current bleach protocol (as of the writing of this
manual, it is BLEACHv2_450) for each of the respective modules
in the Protocol drop-down list. Select all four modules, 1 to 4, and
click Run. The fluidics station will not start the bleach protocol
until the needle lever is pressed down (Figure 6.4).
Temperature will ramp up to 50°C.
7. Follow the prompts on each LCD. Load empty 1.5 mL vials onto
each module if not already done so.
8. Press down on each of the needle levers to start the bleach
protocol (Figure 6.4).