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Mapping 250K/500K SNP assay

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158 GeneChip ® <strong>Mapping</strong> <strong>500K</strong> Assay Manual<br />

the plastic bottle on fluidics station.<br />

The shelf life of this solution is 24 hours. After this period, you must<br />

prepare a fresh solution.<br />

Each fluidics station with four modules requires 500 mL of the<br />

0.525% sodium hypochlorite solution.<br />

3. Place on the fluidics station an empty one liter waste bottle, a<br />

500 mL bottle of bleach and a one liter bottle of DI water as shown<br />

in Figure 6.2. Insert the waste line into the waste bottle (Figure<br />

6.2).<br />

4. Immerse all three wash and water lines of the fluidics station into<br />

the 500 mL of bleach solution (Figure 6.2). DO NOT IMMERSE<br />

THE WASTE LINE INTO THE BLEACH.<br />

The BLEACH protocol requires approximately one liter of DI water.

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