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Mapping 250K/500K SNP assay

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SCANNING THE PROBE ARRAY<br />

150 GeneChip ® <strong>Mapping</strong> <strong>500K</strong> Assay Manual<br />

3. Insert the cartridge into the scanner and test the autofocus to<br />

ensure the spots do not interfere with the focus. If a focus error<br />

message is observed, remove the spot and apply a new spot. Ensure<br />

that the spots lie flat.<br />

Customers using the Autoloader should refer to the Autoloader<br />

User’s Guide.<br />

1. Select Run → Scanner from the menu bar. Alternatively, click the<br />

Start Scan icon in the tool bar.<br />

The Scanner dialog box appears with a drop-down list of all<br />

unscanned experiments.<br />

2. Select the experiment name that corresponds to the probe array<br />

being scanned.<br />

A previously run experiment can also be selected by using the<br />

Include Scanned Experiments option box. After selecting this<br />

option, previously scanned experiments appear in the drop-down<br />

list.<br />

If the experiment name is not seen in the scanner dialog box, open<br />

the workflow monitor, right-click your experiment, and select<br />

“Advance to Scan.” Refer to the GeneChip ® Operating Software<br />

User’s Guide for further information.<br />

3. Click the Load/Eject button and place the array in the scanner.<br />

Only one scan is required for the GeneChip Scanner 3000 7G.<br />

Please do not attempt to manually open or close the GeneChip ®<br />

Scanner 3000 7G scanner door as this may damage the scanner.<br />

4. Once the experiment has been selected, click the Start button.<br />

A dialog box prompts to load the array into the scanner.

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