Mapping 250K/500K SNP assay

chapter 5 | Washing, Staining, and Scanning Arrays 147
Fluidics Station dialog box at the workstation terminal and the
LCD window displays the status of the washing and staining steps.
7. When staining is finished, remove the microcentrifuge vials
containing stain and replace with three empty microcentrifuge
vials as prompted.
8. Remove the probe arrays from the fluidics station modules by first
pressing down the cartridge lever to the eject position.
9. Check the probe array window for large bubbles or air pockets.
• If bubbles are present, the probe array should be filled with
Array Holding Buffer manually, using a pipette. Take out onehalf
of the solution and then manually fill the probe array with
Array Holding Buffer.
• If the probe array has no large bubbles, it is ready to scan on the
GeneChip ® Scanner 3000 7G. Pull up on the cartridge lever to
engage wash block and proceed to Probe Array Scan on page 148.
If a bubble is present, do not return the probe array to the probe
array holder. The probe array must be filled manually with Array
Holding Buffer.
If the arrays cannot be scanned promptly, keep the probe arrays at
4°C and in the dark until ready for scanning. Scan must be
preformed within 24 hours.
If no more samples require washing and staining, shut down the
fluidics station following the procedure outlined in the section,
Shutting Down the Fluidics Station on page 152.