Mapping 250K/500K SNP assay

chapter 5 | Washing, Staining, and Scanning Arrays 145
Add 820 µL of Array Holding Buffer to each microcentrifuge tube.
One tube is needed per module used.
A vial containing Array Holding Buffer must be placed in sample
holder 3 for each module used.
Table 5.5
FS-450 Fluidics Protocol - Antibody Amplification for Mapping Targets
49 Format (Standard)
Mapping 500Kv1_450
Post Hyb Wash #1 6 cycles of 5 mixes/cycle with Wash Buffer A at 25°C
Post Hyb Wash #2 24 cycles of 5 mixes/cycle with Wash Buffer B at 45°C
Stain Stain the probe array for 10 minutes in SAPE solution at
25°C
Post Stain Wash 6 cycles of 5 mixes/cycle with Wash Buffer A at 25°C
2nd Stain Stain the probe array for 10 minutes in Antibody Stain
Solution at 25°C
3rd Stain Stain the probe array for 10 minutes in SAPE solution at
25°C
Final Wash 10 cycles of 6 mixes/cycle with Wash Buffer A at 30°C.
The final holding temperature is 25°C
Filling Array Fill the array with Array Holding Buffer.
Wash Buffer A = non-stringent wash buffer
Wash Buffer B = stringent wash buffer