Mapping 250K/500K SNP assay

144 GeneChip ® Mapping 500K Assay Manual
A vial containing SAPE Stain Solution must be placed in sample
holder 1 for each module used.
Antibody Stain Solution
Table 5.3
Antibody Solution Mix
Components Volume Final Concentration
Stain Buffer 594 µL 1X
0.5 mg/mL biotinylated antibody 6 µL 5 µg/mL
Total 600 µL
Mix well.
A vial containing Antibody Stain Solution must be placed in sample
holder 2 for each module used.
Array Holding Buffer
Table 5.4
Array Holding Buffer
Components Volume
MES Stock Buffer (12X) 8.3 mL
5 M NaCl 18.5 mL
Tween-20 (10%) 0.1 mL
Water 73.1 mL
Total 100 mL