Mapping 250K/500K SNP assay

chapter 5 | Washing, Staining, and Scanning Arrays 143
Preparing the Staining Reagents
Prepare the following reagents. Volumes given are sufficient for one
probe array. Mix well.
Table 5.1
Stain Buffer
Components 1X Final Concentration
H 2O 800.04 µL
SSPE (20X) 360 µL 6X
Tween-20 (3%) 3.96 µL 0.01%
Denhardt’s (50X) 24 µL 1X
Subtotal 1188 µL
Subtotal/2 594 µL
SAPE Stain Solution
Streptavidin Phycoerythrin (SAPE) should be stored in the dark at
4°C, either foil-wrapped or in an amber tube. Remove SAPE from
refrigerator and tap the tube to mix well before preparing stain
solution. Always prepare the SAPE stain solution immediately before
use. Do not freeze either concentrated SAPE or diluted SAPE stain
solution.
Table 5.2
SAPE Solution Mix
Components Volume Final Concentration
Stain Buffer 594 µL 1X
1 mg/mL Streptavidin Phycoerythrin (SAPE) 6.0 µL 10 µg/mL
Total 600 µL
Mix well.