Mapping 250K/500K SNP assay

Reagent Preparation
Wash A: Non-Stringent Wash Buffer
(6X SSPE, 0.01% Tween 20)
For 1000 mL:
300 mL of 20X SSPE
1.0 mL of 10% Tween-20
699 mL of water
Filter through a 0.2 µm filter.
Store at room temperature.
Wash B: Stringent Wash Buffer
(0.6X SSPE, 0.01% Tween 20)
For 1000 mL:
30 mL of 20X SSPE
1.0 mL of 10% Tween-20
969 mL of water
Filter through a 0.2 µm filter.
Store at room temperature.
The pH should be 8.
chapter 5 | Washing, Staining, and Scanning Arrays 137
Prepare Wash B in smaller quantities to avoid long term storage.
The container must be sealed tightly to avoid changes in salt
concentration due to evaporation.
0.5 mg/mL Anti-Streptavidin Antibody
Resuspend 0.5 mg in 1 mL of water.
Store at 4°C.