Mapping 250K/500K SNP assay

128 GeneChip ® Mapping 500K Assay Manual
8. Cut the adhesive film between each row of samples.
Do not remove the film.
9. Place one set of 24 wells onto the thermal cycler and close the lid.
10. Keep the remaining sets of wells in a cooling chamber on ice.
11. Run the 500K Hyb program.
Load the Samples onto Arrays
This procedure requires 2 operators working simultaneously.
Operator 1 loads the samples onto the arrays; Operator 2 covers the
septa with Tough-Spots and loads the arrays into the hybridization
ovens.
To load the samples onto arrays:
Operator 1 Tasks
500K Hyb Program
Temperature Time
95ºC 10 minutes
49ºC Hold
1. When the plate reaches 49 °C, open the lid on the thermal cycler.
2. Remove the film from the first row.
3. Using a single-channel P200 pipette, remove 200 µL of denatured
sample from the first well.
4. Immediately inject the sample into an array.
5. Pass the array to Operator 2.
NOTE: The tasks for Operator 2 are listed below.
6. Remove 200 µL of denatured sample and immediately inject it
into an array.
7. Pass the array to Operator 2.
8. Repeat this process one sample at a time until all 24 samples are
loaded onto arrays.