Mapping 250K/500K SNP assay

Figure 4.8
Applying Tough-Spots ® to the array cartridge
chapter 4 | 96-Well Plate Protocol 127
Method 2 — Using an Applied Biosystems 2720 Thermal
Cycler or an MJ Tetrad PTC-225 Thermal Cycler
For this method, you can use an Applied Biosystems 2720 Thermal
Cycler or an MJ Tetrad PTC-225 Thermal Cycler. The thermal cycler
must be located adjacent to the hybridization ovens. Because the lids
on these thermal cyclers do not slide back, you will process 24 samples
at a time.
Add Hybridization Master Mix and Denature
To add Hybridization Master Mix and denature the samples:
1. Pour 20.9 mL Hybridization Master Mix into a solution basin.
2. Using a 12-channel P200 pipette, add 190 µL of Hybridization
Master Mix to each sample on the Label Plate.
Total volume in each well is 260 µL.
3. IMPORTANT Seal the plate tightly with adhesive film.
4. Vortex the center of the plate for 3 minutes.
5. Cut the plate into 4 strips of two rows each.
6. Put each strip of 24 samples into a plate holder, 2 strips per holder.
7. Spin down the strips at 2000 rpm for 30 sec.