Mapping 250K/500K SNP assay

Load the Samples onto Arrays
chapter 4 | 96-Well Plate Protocol 125
This procedure requires 2 operators working simultaneously.
Operator 1 loads the samples onto the arrays; Operator 2 covers the
septa with Tough-Spots and loads the arrays into the hybridization
ovens.
To load the samples onto arrays:
Operator 1 Tasks
1. When the plate reaches 49 °C, slide back the lid on the thermal
cycler enough to expose the first row of samples only.
2. Remove the film from the first row.
3. Using a single-channel P200 pipette, remove 200 µL of denatured
sample from the first well.
4. Immediately inject the sample into an array.
5. Pass the array to Operator 2.
NOTE: The tasks for Operator 2 are listed below.
6. Remove 200 µL of sample from the next well and immediately
inject it into an array.
7. Pass the array to Operator 2.
8. Repeat this process one sample at a time until the entire row is
loaded.
9. Place a fresh strip of adhesive film over the completed row.
10. Slide the thermal cycler lid back to expose the next row of samples.
11. Repeat steps 3 through 10 until all of the samples have been loaded
onto arrays.