Mapping 250K/500K SNP assay

PREPARE THE ARRAYS
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122 GeneChip ® Mapping 500K Assay Manual
To prepare the arrays:
1. Unwrap the arrays and place on the bench top, septa-side up.
2. Mark each array with a meaningful designation (e.g., a number) to
ensure that you know which sample is loaded onto each array.
3. Allow the arrays to warm to room temperature by leaving on the
bench top 10 to 15 minutes.
4. Insert a 200 µL pipette tip into the upper right septum of each
array.
To ensure that the data collected during scanning is associated with
the correct sample, number the arrays in a meaningful way. It is
critical that you know which sample is loaded onto each array.
PREPARE THE HYBRIDIZATION MASTER MIX
As an option, you can prepare a larger volume of Hybridization Master
Mix than required. The extra mix can be aliquoted and stored at –
20 °C for up to one week.
Preparing Fresh Hybridization Master Mix
To prepare the Hybridization Master Mix:
1. To the 50 mL centrifuge tube, add the reagents in the order shown
in Table 4.37.
DMSO addition: pipette directly into the solution of other
reagents. Avoid pipetting along the side of the tube.
2. Mix well.
3. If making a larger volume, aliquot out 20.9 mL, and store the
remainder at –20 °C for up to one week.