Mapping 250K/500K SNP assay

chapter 4 | 96-Well Plate Protocol 121
An accurate hybridization temperature is critical for this assay.
Therefore, we recommend that your hybridization ovens be
serviced at least once per year to ensure that they are operating
within manufacture specifications.
Thaw Reagents
If the labeled samples from the previous stage were frozen:
1. Thaw the plate on the bench top.
2. Vortex the center of the plate at high speed for 3 sec.
3. Spin down the plate at 2000 rpm for 30 sec.
4. Place in a cooling chamber on ice.
5. If hybridizing samples using Method 1 or 2, the labeled samples
must be placed in a Bio-Rad unskirted 96-well plate
(P/N MLP-9601). For Method 2, the plate will be cut into 4 strips
of 24 wells each.
Preheat the Thermal Cycler Lid
A thermal cycler is required only if you are hybridizing samples using
Method 1 or 2. See Hybridizing Samples Using a Thermal Cycler on
page 124.
Power on the thermal cycler to preheat the lid. Leave the block at room
temperature.
PREHEAT THE HEAT BLOCKS
Heat blocks are required only if you are hybridizing samples using
Method 3. See Hybridizing Samples Using Heat Blocks on page 130.
To prepare the heat blocks:
1. Turn on both heat blocks.
2. Preheat one to 99 °C.
3. Preheat the other to 49 °C.