Mapping 250K/500K SNP assay

120 GeneChip ® Mapping 500K Assay Manual
PREPARE THE REAGENTS, CONSUMABLES AND OTHER COMPONENTS
Prepare a 12X MES Stock Solution
The 12X MES stock solution can be prepared in bulk and kept for at
least one month if properly stored. Proper storage:
• Protect from light using aluminum foil
• Keep at 4 °C
Do not autoclave. Store between 2 °C and 8 °C, and shield from
light using aluminum foil. Discard solution if it turns yellow.
To prepare 1000 mL of 12X MES Stock Solution:
(1.25 M MES, 0.89 M [Na + ])
1. Combine:
• 70.4 g MES hydrate
• 193.3 g MES sodium salt
• 800 mL AccuGENE® water
2. Mix and adjust volume to 1,000 mL.
3. Test the pH.
The pH should be between 6.5 and 6.7.
4. Filter the solution through a 0.2 µm filter.
5. Protect from light using aluminum foil and store at 4 °C.
Preheat the Hybridization Ovens
To preheat the hybridization ovens:
1. Turn each oven on and set the temperature to 49 °C.
2. Set the rpm to 60.
3. Turn the rotation on and allow to preheat for 1 hour before loading
arrays.