Mapping 250K/500K SNP assay

112 GeneChip ® Mapping 500K Assay Manual
Reagent Volume/Rx
Fragmented DNA
(less the 4 µL used for gel analysis)
3. Seal the plate tightly with adhesive film.
4. Vortex the center of the plate at high speed for 3 sec.
5. Spin down the plate at 2000 rpm for 30 sec.
50.5 µL
Labeling Mix 19.5 µL
Total 70 µL
Check to ensure that the plate is tightly sealed, particularly around
the wells on the edge of the plate. The plate must be tightly sealed
to minimize evaporation while on the thermal cycler.
6. Place the plate on the pre-heated thermal cycler block, and run the
500K Label program.
Samples can remain at 4 °C overnight.
500K Label Program
Temperature Time
37ºC 4 hours
95ºC 15 minutes
4ºC Hold
Samples can remain at 4 °C overnight.
7. When the 500K Labeling program is finished:
A. Remove the plate from the thermal cycler.
B. Spin down the plate at 2000 rpm for 30 sec.