Mapping 250K/500K SNP assay

110 GeneChip ® Mapping 500K Assay Manual
Prepare Your Work Area
To prepare the work area:
1. Place a double cooling chamber and a cooler on ice.
2. Prepare the reagents as follows:
A. Vortex each reagent at high speed 3 times, 1 sec each time.
B. Pulse spin for 3 sec.
C. Place in the cooling chamber.
3. Label and place the following in the cooling chamber:
• One strip of 12 tubes labeled MM
• One 15 mL centrifuge tube labeled MM
• Plate of fragmented reactions from the previous stage
Preheat the Thermal Cycler Block
The block must be heated to 37 °C before samples are loaded.
To preheat the thermal cycler block:
1. Turn on the thermal cycler and preheat the block to 37 °C.
2. Allow it to heat for 10 minutes before loading samples.
PREPARE THE LABELING MASTER MIX
Preparation
Keep all reagents and tubes on ice while preparing the Labeling
Master Mix.
To prepare the Labeling Master Mix:
1. Add the following to the 15 mL centrifuge tube on ice using the
volumes shown in Table 4.33:
• 5X TdT Buffer
• GeneChip® DNA Labeling Reagent
2. Remove the TdT enzyme from the freezer and immediately place
in the cooler.