Mapping 250K/500K SNP assay

WHAT YOU CAN DO NEXT
chapter 4 | 96-Well Plate Protocol 105
To minimize solution loss due to evaporation, make sure that the
plate is tightly sealed prior to loading onto the thermal cycler. The
MJ thermal cyclers are more prone to evaporation.
4. Vortex the center of the plate at high speed for 3 sec.
5. Place the plate in a chilled plastic plate holder and spin it down at
4 °C at 2000 rpm for 30 sec.
6. Immediately load the plate onto the pre-heated block of the
thermal cycler (37 °C) and run the 500K Fragment program.
500K Fragment Program
Temperature Time
37ºC 35 minutes
95ºC 15 minutes
4ºC Hold
7. Discard any remaining diluted Fragmentation Reagent.
Diluted Fragmentation Reagent should never be reused.
Proceed directly to the next stage. Concurrently, check the
fragmentation reaction by running gels as described below.