Mapping 250K/500K SNP assay

104 GeneChip ® Mapping 500K Assay Manual
2) Allow to cool on ice.
B. Remove the Fragmentation Reagent from the freezer and:
1) Immediately pulse spin for 3 sec.
Spinning is required because the Fragmentation Reagent
tends to cling to the top of the tube, making it warm
quicker.
2) Immediately place in a cooler.
C. Add the Fragmentation Reagent to the 1.5 mL Eppendorf tube.
D. Vortex the diluted Fragmentation Reagent at high speed
3 times, 1 sec each time.
E. Pulse spin for 3 sec and immediately place on ice.
5. Proceed immediately to the next set of steps, Add Diluted
Fragmentation Reagent to the Samples.
Add Diluted Fragmentation Reagent to the Samples
To add diluted Fragmentation Reagent to the samples:
1. Quickly and on ice, aliquot 50 µL of diluted Fragmentation
Reagent to each tube of the 12 tube strip labeled FR.
2. Using a 12-channel P20 pipette, add 5 µL of diluted
Fragmentation Reagent to each sample.
Do not pipette up and down.
Reagent Volume/Sample
Sample with Fragmentation Buffer 50 µL
Diluted Fragmentation Reagent (0.05 U/µL) 5 µL
Total 55 µL
3. Seal the plate and inspect the edges to ensure that it is tightly
sealed.