Mapping 250K/500K SNP assay

chapter 4 | 96-Well Plate Protocol 103
Table 4.30
Dilution Recipes for Fragmentation Reagent Concentrations of 2 and 3 U/µL
Reagent
2. If the concentration is 2 or 3 U/µL, dilute the Fragmentation
Reagent using the volumes show in Table 4.30 above.
3. If the concentration is not 2 or 3 U/µL, use the formula below to
calculate the dilution required to bring the reagent to a final
concentration of 0.05 U/µL.
4. Dilute the Fragmentation Reagent to 0.05 U/µL as follows using
the recipes in Table 4.30 or the dilution formula calculation:
A. To the 1.5 mL Eppendorf tube on ice:
Fragmentation Reagent
Concentration
2 U/µL 3U/µL
AccuGENE® water 525 µL 530 µL
Fragmentation Buffer 60 µL 60 µL
Fragmentation Reagent 15 µL 10 µL
Total
(enough for 96 samples)
Formula Y = 0.05 U/µL * 600 µL
X U/µL
Where:
600 µL 600 µL
Y = number of µL of stock Fragmentation Reagent
X = number of U of stock Fragmentation Reagent per µL
(per label on tube)
0.05 U/µL = final concentration of Fragmentation Reagent
600 µL = final volume of diluted Fragmentation Reagent
(enough for 96 reactions)
1) Add the AccuGENE® water and Fragmentation Buffer.